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KMID : 0381920060360030141
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Korean Journal of Microscopy
2006 Volume.36 No. 3 p.141 ~ p.153
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The Cytotoxicity and Effect on the Experimental Rabbit Cornea of Soft Contact Lens Multi-Purpose Solution (MPS)
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Koh Eun-Kyung
Chae Soo-Chul
Park Soo-Kyung
Kim Deok-Song
Lee Jong-Bin
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Abstract
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The purpose of this study is to find out how soft contact lens multi-purpose solution (MPS), often used for medical treatment, effects the inhibition on cell growth and research the result of using MPS, suspected to demage eye cells, on rabbit eye’s corneal epithelium and endothelium tissue. In this treatise, ReNu® (Baush & Lomb, USA), Opti-free express® (Alcon, USA), Free-sol plus® (Hanamedicon, Korea) had been selected among the MPS. After culturing L929 cell line, cell growth inhibition rate was measured by MTT assay, and by making Hematoxylin and Eosin stain specimen, the orphology was observed by optical microscope. In the In vivo experiment, 9 white rabbit eyes (18 eyes) were classified into 3 roups. The experimental group is left eyes (9 eyes) of rabbit, and MPS were dropped; however, the control group, the right eyes (9 eyes), were only used a saline solution without preservatives. After the dropping within the period, the cornea surface of rabbit eyes were stained by Rose bengal and observed. To figure out the changes of the corneal epithelium and dothelium tissue scanning electron microscopy (SEM) has been used. As the result, the rate of cell growth inhibition was 54%, 73% and 36%, respectively. Morphological changes represented that the shape has been changed into oval or round shape and those are not considered as a common formation of L929 cell line. When it comes to staining Rose bengal, each xperiment group was stained red which is not shown in controls. The polygonal mosaic pattern of a corneal epithelium was isturbed in the picture taken by SEM; furthermore, the shape of the corneal endothelium was irregular. In conclusion, as we onsider antimicrobial effect and the safety on living cells, it is necessary that we should improve concentration of preservatives and study ontinuously to develop a new preservatives without a toxic effect on the cornea surface.
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KEYWORD
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Rabbit cornea, Cytotoxicity, Soft contact lens Multi-Purpose Solution (MPS), Preservatives
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